Sections
Title | Starting Page | Number of Pages |
---|
Table of Contents | 5 | 4 |
List of Tables | 7 | 1 |
List of Figures | 8 | 1 |
Cell Culture - Introduction | 9 | 1 |
Introduction | 9 | 1 |
Cell Culture - Overview | 10 | 19 |
Overview | 10 | 1 |
Cell Lines | 10 | 1 |
Application of Cell Lines | 10 | 1 |
Characteristics of Cell Lines | 10 | 1 |
Selective Cell Lines | 11 | 1 |
Chinese Hamster Ovary | 11 | 1 |
SP2/0 and NS0 | 11 | 1 |
HEK 293 and PER. C6 | 11 | 1 |
Transformational Approach of Transgenic Model | 12 | 1 |
Transgenic DNA Preparation | 12 | 1 |
Insertion of the Transgenic DNA into Animals | 12 | 1 |
Clone and Integrity Verification of the Transgene | 13 | 1 |
DNA Purification and Establishing a Screening Method | 13 | 1 |
Breeding and Analysis of Transgenic Rodents | 13 | 1 |
Stem Cell Research has Enormous Potential for Cell Culture | 14 | 1 |
Applications of Stem Cell Research | 14 | 1 |
Stem Cell Research Market Analysis | 15 | 1 |
Industry Rivalry | 15 | 1 |
Threat of New Entrants | 15 | 1 |
Threat of Substitutes | 16 | 1 |
Supplier Power | 16 | 1 |
Buyer Power | 16 | 1 |
Fermentation Methods | 16 | 1 |
Fed-Batch Fermentation | 17 | 1 |
Suspended versus Anchored Cells | 18 | 1 |
Microencapsulation of Cells | 18 | 1 |
Important Factors for Regulating Cell Culture | 18 | 1 |
Components of Basal Media | 18 | 1 |
pH | 18 | 1 |
Osmotic Pressure | 19 | 1 |
Energy Sources | 19 | 1 |
Nutrients | 19 | 1 |
Fermenters and Bioreactors | 19 | 1 |
Bioreactor | 19 | 1 |
Selection of Bioreactors | 19 | 1 |
Batch Bioreactor | 20 | 1 |
Characteristics | 20 | 1 |
Applications | 20 | 1 |
Continuous Bioreactor | 21 | 1 |
Key Features | 21 | 1 |
Characteristics | 21 | 1 |
Common Applications | 22 | 1 |
Submerged Bioreactors Stirred Tank | 22 | 1 |
Airlift Bioreactor | 22 | 1 |
Characteristics | 22 | 1 |
Summary | 22 | 1 |
Water-Jacketed Bioreactor | 23 | 1 |
Summary | 23 | 1 |
Characteristics | 23 | 1 |
Media | 23 | 1 |
Role of Media | 23 | 1 |
Types of Media | 23 | 1 |
Lysogeny Broth | 23 | 1 |
Serum Free Media | 23 | 1 |
Protein-Free Media | 24 | 1 |
Specialty Media | 24 | 1 |
Sera | 24 | 1 |
Sources of Sera | 24 | 1 |
Sera Safety and Regulations | 25 | 1 |
Shift to Serum Free Media | 26 | 1 |
Reagents | 26 | 1 |
Major Types of Biologic Reagents | 26 | 1 |
Amino Acids | 26 | 1 |
Growth Factors | 26 | 1 |
Albumin | 26 | 1 |
Transferrin | 26 | 1 |
Thrombin | 27 | 1 |
Growth Supplements | 27 | 1 |
Protease Inhibitor | 27 | 1 |
Attachment Factors | 27 | 1 |
Growing Trend for Non-animal Derived Reagents | 27 | 1 |
Reagent Safety Issues | 28 | 1 |
Cell Culture - Current and Future Trends | 29 | 6 |
Cell Culture Market Forecast | 29 | 1 |
Media Market Forecast | 30 | 1 |
Sera Market Forecast | 31 | 1 |
Reagents Market Forecast | 32 | 1 |
Drivers and Restraints for Cell Culture Market | 33 | 1 |
Drivers for Cell Culture Market | 33 | 1 |
Increasing Demand for Biopharmaceuticals | 33 | 1 |
Emerging Countries are expected to contribute more to Biosimilars Market | 33 | 1 |
Restraints for Cell Culture Market | 34 | 1 |
Low Production Flexibility | 34 | 1 |
Cell Culture - Biopharmaceuticals an Important Part of Cell Culturing | 35 | 10 |
Genetic Modifications | 35 | 1 |
Cell Culture Life-Cycle Expressions | 35 | 1 |
Use of Alternative Approaches to Improve Protein Expression System | 35 | 1 |
Disposable Technologies to Reduce the Process Time | 36 | 1 |
Disposable Technology in Vaccine Manufacturing | 36 | 1 |
Biotechnological Product Development Using Cell Culture Method | 37 | 1 |
Overview of Microbial Fermentation | 37 | 1 |
Overview of Mammalian Cell Culture Systems | 37 | 1 |
Cell Banking and Seed Culture | 38 | 1 |
Research Cell Banks | 38 | 1 |
Master Cell Banks | 38 | 1 |
Working Cell Banks | 39 | 1 |
Cell Systems | 39 | 1 |
Animal Cell Systems | 39 | 1 |
Insect Cell Systems | 39 | 1 |
Bacteria and Yeast Systems | 40 | 1 |
Advantages and Disadvantages of Cell Culture Techniques | 41 | 1 |
Advantages: | 41 | 1 |
Disadvantages: | 41 | 1 |
Cell Lines and Banks | 41 | 1 |
Manufacturing of Biopharmaceuticals using Cell Culture Techniques | 41 | 1 |
Manufacturing Challenges | 42 | 1 |
Standard Methods for Cell Culture | 42 | 1 |
Validation and Quality Maintenance | 43 | 1 |
Essential Guidelines for Maintaining Sterilization Standards | 43 | 1 |
Filling Line Sterilization | 44 | 1 |
Successful Treatments from Biopharmaceuticals is Increasing the Demand for Cell Culturing | 44 | 1 |
Key Players in the Culture Media, Sera and Reagents Market | 45 | 27 |
Life Technologies Corporation | 46 | 1 |
Sigma-Aldrich Corporation | 47 | 1 |
Thermo Fisher Scientific Inc. | 48 | 1 |
Affymetrix, Inc. | 49 | 1 |
BD Biosciences | 50 | 1 |
EMD Millipore | 51 | 1 |
Advanced Biotechnologies, Inc. | 52 | 1 |
Amresco | 53 | 1 |
Analytical Biological Services Inc. | 54 | 1 |
Athena Biotechnologies, Inc. | 55 | 1 |
Biochrom AG | 56 | 1 |
Bioreclamation, Inc. | 57 | 1 |
Cell Essentials, Inc | 58 | 1 |
CellGenix Technologie Transfer GmbH | 59 | 1 |
Clontech Laboratories, Inc. | 60 | 1 |
Gemini Bio-Products | 61 | 1 |
Lonza BioSciences Singapore Pte Ltd | 62 | 1 |
Mediatech, Inc. | 63 | 1 |
MP Biomedicals, LLC | 64 | 1 |
PAA Laboratories GmbH | 65 | 1 |
Quality Biological, Inc. | 66 | 1 |
R&D Systems, Inc. | 67 | 1 |
SAFC Biosciences, Inc. | 68 | 1 |
Selexis SA | 69 | 1 |
SeraCare Life Sciences, Inc. | 70 | 1 |
Sera Laboratories International Ltd. | 71 | 1 |
Cell Culture - Appendix | 72 | 4 |
Market Definitions | 72 | 1 |
Abbreviations | 72 | 1 |
Bibliography | 73 | 1 |
Research Methodology | 74 | 2 |
Coverage | 74 | 1 |
Secondary Research | 74 | 1 |
Primary Research | 75 | 1 |
Expert Panel Validation | 75 | 1 |
Contact Us | 75 | 1 |
Disclaimer | 75 | 1 |