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Sections |
| Title | Starting Page | Number of Pages |
|---|
| Table of Contents
Advances in the use of biomarkers in biochip and microarray testing
Executive Summary 12
Biochips, biomarkers, and in vitro diagnostics 12
DNA microarrays 13
Protein microarrays 14
Tissue and cell microarrays 16
Lab-on-a-Chip technologies | 0 | 1 |
| Table of Contents | 0 | 1 |
| Advances in the use of biomarkers in biochip and microarray testing | 0 | 1 |
| Executive Summary 12 | 0 | 1 |
| Biochips, biomarkers, and in vitro diagnostics 12 | 0 | 1 |
| DNA microarrays 13 | 0 | 1 |
| Protein microarrays 14 | 0 | 1 |
| Tissue and cell microarrays 16 | 0 | 1 |
| Lab-on-a-Chip technologies 17 | 0 | 1 |
| Biochip companies 18 | 0 | 1 |
| Market Analysis and Forecasts 18 | 0 | 1 |
| Chapter 1 Biochips, biomarkers, and in vitro diagnostics 22 | 0 | 1 |
| Summary 22 | 0 | 1 |
| Biomarker-based IVDs 23 | 0 | 1 |
| Introduction to biochips 23 | 0 | 1 |
| Microarrays 23 | 0 | 1 |
| Microfluidic chips 24 | 0 | 1 |
| Background on nucleic acid and protein testing 25 | 0 | 1 |
| Immunoassay technology 25 | 0 | 1 |
| Immunoassay formats 26 | 0 | 1 |
| Rapid immunochromatographic assays 28 | 0 | 1 |
| Multiplexed assays 28 | 0 | 1 |
| Nucleic acid testing 29 | 0 | 1 |
| Probe-based NAT 30 | 0 | 1 |
| Amplification technologies 32 | 0 | 1 |
| Biomarker discovery and validation 33 | 0 | 1 |
| Translating biomarkers into IVDs 35 | 0 | 1 |
| Regulation of in vitro diagnostics 36 | 0 | 1 |
| Europe 37 | 0 | 1 |
| USA 37 | 0 | 1 |
| Draft guidance on multivariate analysis 40 | 0 | 1 |
| Chapter 2 DNA microarrays 44 | 0 | 1 |
| Summary 44 | 0 | 1 |
| Introduction 46 | 0 | 1 |
| DNA microarray fabrication technologies 49 | 0 | 1 |
| Standard microarrays 49 | 0 | 1 |
| 3-D DNA microarrays 53 | 0 | 1 |
| Particle (bead)-based microarrays 54 | 0 | 1 |
| Analytical uses of DNA microarrays 56 | 0 | 1 |
| SNP genotyping 56 | 0 | 1 |
| Detection of genetic imbalances 57 | 0 | 1 |
| Gene expression profiling 59 | 0 | 1 |
| Single-channel microarrays 60 | 0 | 1 |
| Two-channel microarrays 62 | 0 | 1 |
| Methods utilizing RT-PCR 64 | 0 | 1 |
| MicroRNA expression profiling 65 | 0 | 1 |
| Limitations and barriers to commercialization 66 | 0 | 1 |
| Key commercial players 69 | 0 | 1 |
| DNA microarray testing in cancer 70 | 0 | 1 |
| Overview 71 | 0 | 1 |
| Progress in breast cancer 74 | 0 | 1 |
| Early detection 74 | 0 | 1 |
| Classification, prognosis, and treatment 75 | 0 | 1 |
| Tumors of unidentified origin 79 | 0 | 1 |
| Other advances 80 | 0 | 1 |
| DNA microarray testing in genetic disorders 82 | 0 | 1 |
| Microarrays testing for adverse drug reactions 86 | 0 | 1 |
| DNA microarray testing in transplant management 87 | 0 | 1 |
| DNA microarray testing in other conditions 88 | 0 | 1 |
| Expanding applications 91 | 0 | 1 |
| Chapter 3 Protein microarrays 94 | 0 | 1 |
| Summary 94 | 0 | 1 |
| Introduction 96 | 0 | 1 |
| Background on proteomics 97 | 0 | 1 |
| Protein microarray technologies 100 | 0 | 1 |
| Formats 102 | 0 | 1 |
| Glass slides 102 | 0 | 1 |
| Microwell/nanowell chips 103 | 0 | 1 |
| Implementation 103 | 0 | 1 |
| 3.3.2.1 Forward-phase microarrays 103 | 0 | 1 |
| Prospects for multiplex ELISAs 104 | 0 | 1 |
| Reverse-phase microarrays 105 | 0 | 1 |
| Detection of bound targets 106 | 0 | 1 |
| SELDI ProteinChip technology 107 | 0 | 1 |
| Fabrication technologies 109 | 0 | 1 |
| Dispensing technology 109 | 0 | 1 |
| Protein immobilization strategies 110 | 0 | 1 |
| Surface chemistries 111 | 0 | 1 |
| Analytical uses of protein microarrays 113 | 0 | 1 |
| Commercial microarrays for clinical research 114 | 0 | 1 |
| Protein microarray diagnostics in cancer 118 | 0 | 1 |
| Chapter 4 Tissue and cell microarrays 124 | 0 | 1 |
| Summary 124 | 0 | 1 |
| Introduction 125 | 0 | 1 |
| Tissue microarrays 127 | 0 | 1 |
| Advantages 127 | 0 | 1 |
| Construction 128 | 0 | 1 |
| Sources of tissue 129 | 0 | 1 |
| Scanning and analysis 130 | 0 | 1 |
| Companies providing products and services 131 | 0 | 1 |
| Uses of tissue microarrays 132 | 0 | 1 |
| Clinical investigations in cancer 134 | 0 | 1 |
| Initiatives using cell microarrays 135 | 0 | 1 |
| Overview 135 | 0 | 1 |
| Examples of diagnostic applications 137 | 0 | 1 |
| Chapter 5 Lab-on-a-Chip Technologies 140 | 0 | 1 |
| Summary 140 | 0 | 1 |
| Introduction to microfluidics 141 | 0 | 1 |
| Lab-on-a-chip devices 144 | 0 | 1 |
| Construction methods 145 | 0 | 1 |
| Companies and technologies 146 | 0 | 1 |
| Multiplexed particle assays 150 | 0 | 1 |
| Multiplexing at the microlevel 151 | 0 | 1 |
| Particle encoding technologies 152 | 0 | 1 |
| Applications of LoC devices 153 | 0 | 1 |
| Changing diagnostics 154 | 0 | 1 |
| Future role in PoC testing 155 | 0 | 1 |
| Chapter 6 Biochip companies 160 | 0 | 1 |
| Introduction/Summary 160 | 0 | 1 |
| Accelr8 Technology Corporation 160 | 0 | 1 |
| Company description 160 | 0 | 1 |
| Microarray-based rapid bacterial analysis 161 | 0 | 1 |
| Advanced Liquid Logic Inc 162 | 0 | 1 |
| Company description 162 | 0 | 1 |
| Digital Microfluidics devices 163 | 0 | 1 |
| Affymetrix Inc 164 | 0 | 1 |
| Company description 164 | 0 | 1 |
| DNA and protein microarrays 165 | 0 | 1 |
| Agendia BV 167 | 0 | 1 |
| Company description 167 | 0 | 1 |
| Gene expression microarray diagnostics 167 | 0 | 1 |
| MammaPrint 167 | 0 | 1 |
| TargetPrint 168 | 0 | 1 |
| CupPrint 168 | 0 | 1 |
| ColoPrint 168 | 0 | 1 |
| Agilent Technologies Inc 169 | 0 | 1 |
| Company description 169 | 0 | 1 |
| DNA microarrays 169 | 0 | 1 |
| Almac Group Ltd 170 | 0 | 1 |
| Company description 170
Microarray-based gene expression profiling 171
Ambry Genetics Corp 171
Company description 171
Microarray-based cytogenetic testing 172
Applied Microarrays Inc 172
Company description 173
3-D DNA microarrays 173
Arrayit Corp 174
Com | 0 | 1 |
